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Cortical Actomyosin Flows are Generated by Actomyosin Cluster Vibrations in Vitro

Sven K. Vogel, Christian Wölfer, Diego A. Ramirez, Robert J. Flassig, Kai Sundmacher, Petra Schwille
doi: https://doi.org/10.1101/394700
Sven K. Vogel
1Max-Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
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Christian Wölfer
2Process Synthesis and Process Dynamics and Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, D-39106 Magdeburg, Germany
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Diego A. Ramirez
1Max-Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
4Graduate School of Quantitative Biosciences, Ludwig-Maximilians-University, Feodor-Lynen-Str. 25, D-81377 Munich, Germany
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Robert J. Flassig
3Process Systems Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, D-39106 Magdeburg, Germany
5Brandenburg University of Applied Science, Magdeburger Straße 50, D-14770 Brandenburg, Germany
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Kai Sundmacher
3Process Systems Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, D-39106 Magdeburg, Germany
6Department of Process Systems Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, D-39106 Magdeburg, Germany
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Petra Schwille
1Max-Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
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Abstract

Cortical actomyosin dynamics and flows play pivotal roles in eukaryotic cells, e.g. for cell motility, cell division and chiral morphogenesis during early embryogenesis of multicellular organisms. For many model systems myosin motors have been proposed to play a key role in the generation of cortical actomyosin dynamics and flows. However, little is known about the mechanisms behind these actomyosin flows. We reconstituted and confined minimal actin cortices inside water in oil droplets. These spherically confined actomyosin cortices exhibit directional actomyosin flow-like motions upon ATP induced contractility of the myosin motors. By combining our experiments with a theoretical description, we found that the observed direct motion of the actomyosin clusters arises from vibrations within the individual actomyosin clusters. By tracking actin clusters, we detected fingerprints of vibrational states driving their directed motions in the spherical confinement. These vibrations may provide a mechanism driving cortical actomyosin dynamics and flows in living systems.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 18, 2018.
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Cortical Actomyosin Flows are Generated by Actomyosin Cluster Vibrations in Vitro
Sven K. Vogel, Christian Wölfer, Diego A. Ramirez, Robert J. Flassig, Kai Sundmacher, Petra Schwille
bioRxiv 394700; doi: https://doi.org/10.1101/394700
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Cortical Actomyosin Flows are Generated by Actomyosin Cluster Vibrations in Vitro
Sven K. Vogel, Christian Wölfer, Diego A. Ramirez, Robert J. Flassig, Kai Sundmacher, Petra Schwille
bioRxiv 394700; doi: https://doi.org/10.1101/394700

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