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Multipoint and large volume fiber photometry with a single tapered optical fiber implant

View ORCID ProfileFilippo Pisano, View ORCID ProfileMarco Pisanello, Emanuela Maglie, Antonio Balena, Leonardo Sileo, View ORCID ProfileBarbara Spagnolo, View ORCID ProfileMinsuk Hyun, View ORCID ProfileMassimo De Vittorio, View ORCID ProfileBernardo L. Sabatini, View ORCID ProfileFerruccio Pisanello
doi: https://doi.org/10.1101/455766
Filippo Pisano
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
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Marco Pisanello
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
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Emanuela Maglie
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
2Dip. di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy.
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Antonio Balena
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
2Dip. di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy.
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Leonardo Sileo
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
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Barbara Spagnolo
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
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Minsuk Hyun
3Dept. of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, 02115 MA, U.S.A.
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Massimo De Vittorio
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
2Dip. di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy.
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Bernardo L. Sabatini
3Dept. of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, 02115 MA, U.S.A.
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Ferruccio Pisanello
1Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, 73010 Arnesano (LE), Italy.
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Abstract

Techniques to monitor functional fluorescence signal from the brain are increasingly popular in the neuroscience community. However, most implementations are based on flat cleaved optical fibers (FFs) that can only interface with shallow tissue volumes adjacent to the fiber opening. To circumvent this limitation, we exploit modal properties of tapered optical fibers (TFs) to structure light collection over the wide optically active area of the fiber taper, providing an approach to efficiently and selectively collect light from the region(s) of interest. While being less invasive than FFs, TF probes can uniformly collect light over up to 2 mm of tissue and allow for multisite photometry along the taper. Furthermore, by micro-structuring the non-planar surface of the fiber taper, collection volumes from TFs can also be engineered arbitrarily in both shape and size. Owing to the abilities offered by these probes, we envision that TFs can set a novel, powerful paradigm in optically targeting not only the deep brain, but, more in general, any biological system or organ where light collection from the deep tissues is beneficial but challenging because of tissue scattering and absorption.

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Posted October 29, 2018.
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Multipoint and large volume fiber photometry with a single tapered optical fiber implant
Filippo Pisano, Marco Pisanello, Emanuela Maglie, Antonio Balena, Leonardo Sileo, Barbara Spagnolo, Minsuk Hyun, Massimo De Vittorio, Bernardo L. Sabatini, Ferruccio Pisanello
bioRxiv 455766; doi: https://doi.org/10.1101/455766
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Multipoint and large volume fiber photometry with a single tapered optical fiber implant
Filippo Pisano, Marco Pisanello, Emanuela Maglie, Antonio Balena, Leonardo Sileo, Barbara Spagnolo, Minsuk Hyun, Massimo De Vittorio, Bernardo L. Sabatini, Ferruccio Pisanello
bioRxiv 455766; doi: https://doi.org/10.1101/455766

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