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Squidpy: a scalable framework for spatial single cell analysis

View ORCID ProfileGiovanni Palla, View ORCID ProfileHannah Spitzer, View ORCID ProfileMichal Klein, View ORCID ProfileDavid Fischer, View ORCID ProfileAnna Christina Schaar, View ORCID ProfileLouis Benedikt Kuemmerle, View ORCID ProfileSergei Rybakov, View ORCID ProfileIgnacio L. Ibarra, View ORCID ProfileOlle Holmberg, View ORCID ProfileIsaac Virshup, View ORCID ProfileMohammad Lotfollahi, View ORCID ProfileSabrina Richter, View ORCID ProfileFabian J. Theis
doi: https://doi.org/10.1101/2021.02.19.431994
Giovanni Palla
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
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Hannah Spitzer
1Institute of Computational Biology, Helmholtz Center Munich, Germany
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  • ORCID record for Hannah Spitzer
Michal Klein
1Institute of Computational Biology, Helmholtz Center Munich, Germany
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  • ORCID record for Michal Klein
David Fischer
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
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Anna Christina Schaar
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
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Louis Benedikt Kuemmerle
1Institute of Computational Biology, Helmholtz Center Munich, Germany
4Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Center Munich, Germany
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Sergei Rybakov
1Institute of Computational Biology, Helmholtz Center Munich, Germany
3Department of Mathematics, Technical University of Munich, Germany
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Ignacio L. Ibarra
1Institute of Computational Biology, Helmholtz Center Munich, Germany
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Olle Holmberg
1Institute of Computational Biology, Helmholtz Center Munich, Germany
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Isaac Virshup
5Department of Anatomy and Physiology, University of Melbourne, Australia
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Mohammad Lotfollahi
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
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Sabrina Richter
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
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Fabian J. Theis
1Institute of Computational Biology, Helmholtz Center Munich, Germany
2TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
3Department of Mathematics, Technical University of Munich, Germany
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  • ORCID record for Fabian J. Theis
  • For correspondence: fabian.theis@helmholtz-muenchen.de
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Abstract

Spatial omics data are advancing the study of tissue organization and cellular communication at an unprecedented scale. Here, we present Squidpy, a Python framework that brings together tools from omics and image analysis to enable scalable description of spatial molecular data, such as transcriptome or multivariate proteins. Squidpy provides both infrastructure and numerous analysis methods that allow to efficiently store, manipulate and interactively visualize spatial omics data.

Competing Interest Statement

F.J.T. reports receiving consulting fees from Roche Diagnostics GmbH and Cellarity Inc., and ownership interest in Cellarity, Inc. and Dermagnostix

Footnotes

  • https://github.com/theislab/squidpy

  • https://squidpy.readthedocs.io/en/latest/

  • https://github.com/theislab/squidpy_reproducibility

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 20, 2021.
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Squidpy: a scalable framework for spatial single cell analysis
Giovanni Palla, Hannah Spitzer, Michal Klein, David Fischer, Anna Christina Schaar, Louis Benedikt Kuemmerle, Sergei Rybakov, Ignacio L. Ibarra, Olle Holmberg, Isaac Virshup, Mohammad Lotfollahi, Sabrina Richter, Fabian J. Theis
bioRxiv 2021.02.19.431994; doi: https://doi.org/10.1101/2021.02.19.431994
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Squidpy: a scalable framework for spatial single cell analysis
Giovanni Palla, Hannah Spitzer, Michal Klein, David Fischer, Anna Christina Schaar, Louis Benedikt Kuemmerle, Sergei Rybakov, Ignacio L. Ibarra, Olle Holmberg, Isaac Virshup, Mohammad Lotfollahi, Sabrina Richter, Fabian J. Theis
bioRxiv 2021.02.19.431994; doi: https://doi.org/10.1101/2021.02.19.431994

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