Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Transverse sinus injections: A novel method for whole-brain vector-driven gene delivery

View ORCID ProfileAli S. Hamodi, Aude Martinez Sabino, N. Dalton Fitzgerald, View ORCID ProfileMichael C. Crair
doi: https://doi.org/10.1101/579730
Ali S. Hamodi
1Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ali S. Hamodi
Aude Martinez Sabino
1Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA
2University of Technology of Compiègne, Compiègne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
N. Dalton Fitzgerald
1Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael C. Crair
1Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael C. Crair
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

A major challenge in neuroscience is convenient whole-brain delivery of transgenes, especially early in postnatal development. Recent advances demonstrate whole-brain gene delivery by retro-orbital injection of virus, but slow and sparse expression, and the large injection volumes required, precludes early developmental studies. We developed a novel method for simple, fast and efficient gene delivery across the central nervous system in neonates as early as P4 and persisting into adulthood. The method employs transverse sinus injections of 2-4μL of AAV9 at P0. Here, we describe how to use this method to label and/or genetically manipulate cells in the neonatal rat and mouse brain. This protocol is fast, easy, can be readily adopted by any laboratory, and utilizes the widely available AAV9 capsid. The procedure outlined here is adaptable for diverse experimental applications ranging from biochemistry, anatomical and functional mapping, to gene expression, silencing, and editing.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 16, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Transverse sinus injections: A novel method for whole-brain vector-driven gene delivery
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Transverse sinus injections: A novel method for whole-brain vector-driven gene delivery
Ali S. Hamodi, Aude Martinez Sabino, N. Dalton Fitzgerald, Michael C. Crair
bioRxiv 579730; doi: https://doi.org/10.1101/579730
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Transverse sinus injections: A novel method for whole-brain vector-driven gene delivery
Ali S. Hamodi, Aude Martinez Sabino, N. Dalton Fitzgerald, Michael C. Crair
bioRxiv 579730; doi: https://doi.org/10.1101/579730

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4395)
  • Biochemistry (9619)
  • Bioengineering (7111)
  • Bioinformatics (24915)
  • Biophysics (12642)
  • Cancer Biology (9979)
  • Cell Biology (14388)
  • Clinical Trials (138)
  • Developmental Biology (7968)
  • Ecology (12135)
  • Epidemiology (2067)
  • Evolutionary Biology (16010)
  • Genetics (10938)
  • Genomics (14764)
  • Immunology (9889)
  • Microbiology (23719)
  • Molecular Biology (9493)
  • Neuroscience (50965)
  • Paleontology (370)
  • Pathology (1544)
  • Pharmacology and Toxicology (2688)
  • Physiology (4031)
  • Plant Biology (8685)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2403)
  • Systems Biology (6446)
  • Zoology (1346)