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An organ-on-a-chip approach for investigating root-environment interactions in heterogeneous conditions

View ORCID ProfileClaire E. Stanley, Jagriti Shrivastava, Rik Brugman, Dirk van Swaay, View ORCID ProfileGuido Grossmann
doi: https://doi.org/10.1101/126987
Claire E. Stanley
ETH Zurich;
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Jagriti Shrivastava
Heidelberg University
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Rik Brugman
Heidelberg University
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Dirk van Swaay
ETH Zurich;
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Guido Grossmann
Heidelberg University
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  • For correspondence: guido.grossmann@cos.uni-heidelberg.de
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Abstract

Plants adapt their root morphology in response to changing environmental conditions, yet it remains largely unknown to what extent developmental adaptations are based on systemic or cell-autonomous responses. We present the dual-flow-RootChip (dfRootChip), a microfluidic organ-on-a-chip platform for asymmetric perfusion of Arabidopsis roots to investigate root-environment interactions under simulated environmental heterogeneity. Applications range from root guidance, monitoring of physiology and development under asymmetric conditions, tracing molecular uptake and selective drug treatments to local inoculation with microbes. We measured calcium responses in roots treated with biotic and abiotic elicitors and observed elicitor-specific signal propagation across the root from treated to untreated cells. We provide evidence for non-autonomous positive regulation of hair growth across the root upon exposure to unfavourable conditions on the opposite side. Our approach sheds light on lateral coordination of morphological adaptation and facilitates studies on root physiology, signalling and development in heterogeneous environments at the organ level.

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Posted April 12, 2017.
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An organ-on-a-chip approach for investigating root-environment interactions in heterogeneous conditions
Claire E. Stanley, Jagriti Shrivastava, Rik Brugman, Dirk van Swaay, Guido Grossmann
bioRxiv 126987; doi: https://doi.org/10.1101/126987
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An organ-on-a-chip approach for investigating root-environment interactions in heterogeneous conditions
Claire E. Stanley, Jagriti Shrivastava, Rik Brugman, Dirk van Swaay, Guido Grossmann
bioRxiv 126987; doi: https://doi.org/10.1101/126987

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