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Tolerance to PVY infection in potato is conditioned by perturbation of small RNA-gibberellin regulatory network

Maja Križnik, Marko Petek, David Dobnik, Živa Ramšak, Špela Baebler, Stephan Pollmann, Jan Kreuze, Jana Žel, Kristina Gruden
doi: https://doi.org/10.1101/130757
Maja Križnik
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
2Jožef Stefan International Postgraduate School, Ljubljana, Slovenia
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  • For correspondence: maja.kriznik@nib.si
Marko Petek
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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David Dobnik
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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Živa Ramšak
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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Špela Baebler
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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Stephan Pollmann
3Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, Madrid, Spain.
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Jan Kreuze
4Global Program of Integrated Crop and Systems Research, International Potato Center (CIP), Lima, Peru
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Jana Žel
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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Kristina Gruden
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
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Abstract

Potato virus Y (PVY) is the most economically important potato viral pathogen worldwide. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We identified and quantified the endogenous miRNAs and phasiRNAs as well as virus-derived sRNAs. We constructed a sRNA regulatory network connecting sRNAs and their targets identified to link sRNA level responses to physiological processes. We discovered an interesting novel sRNAs – gibberellin (GA) regulatory circuit being activated as early as 3 days post inoculation, before viral multiplication can be detected. Increased levels of miR167 and phasiRNA931 were reflected in decreased levels of transcripts involved in GA biosynthesis. Moreover, decreased concentration of GA confirmed this regulation. We have additionally showed that this regulation is salicylic acid dependent as the response of sRNA network was attenuated in salicylic acid-depleted transgenic counterpart NahG-Désirée expressing severe disease symptoms. Moreover, we found that differentially expressed miR6022, which targets leucine-rich-repeat receptor-like kinases, likely links GA signaling with immune responses. Besides downregulation of GA signaling, regulation of several other parts of sRNA network in tolerant Désirée revealed striking similarities to responses observed in mutualistic symbiotic interactions. The intertwining of different regulatory networks revealed here shows how developmental signaling, symptomology and stress signaling are balanced.

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Posted April 26, 2017.
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Tolerance to PVY infection in potato is conditioned by perturbation of small RNA-gibberellin regulatory network
Maja Križnik, Marko Petek, David Dobnik, Živa Ramšak, Špela Baebler, Stephan Pollmann, Jan Kreuze, Jana Žel, Kristina Gruden
bioRxiv 130757; doi: https://doi.org/10.1101/130757
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Tolerance to PVY infection in potato is conditioned by perturbation of small RNA-gibberellin regulatory network
Maja Križnik, Marko Petek, David Dobnik, Živa Ramšak, Špela Baebler, Stephan Pollmann, Jan Kreuze, Jana Žel, Kristina Gruden
bioRxiv 130757; doi: https://doi.org/10.1101/130757

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