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Relevance of host cell surface glycan structure for cell specificity of influenza A virus

View ORCID ProfileMarkus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Dandan Zhang, Jianping Liu, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann, View ORCID ProfileChristian Sieben
doi: https://doi.org/10.1101/203349
Markus Kastner
1Institute for Biophysics, Johannes Kepler University Linz, 4020 Linz, Austria
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  • ORCID record for Markus Kastner
Andreas Karner
1Institute for Biophysics, Johannes Kepler University Linz, 4020 Linz, Austria
2Center for Advanced Bioanalysis GmbH, A-4020 Linz, Austria
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Rong Zhu
1Institute for Biophysics, Johannes Kepler University Linz, 4020 Linz, Austria
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Qiang Huang
3State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shangha 200433, China
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Dandan Zhang
4Shanghai Supercomputer Center, Shanghai 201203, China
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Jianping Liu
3State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shangha 200433, China
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Andreas Geissner
5Department for Biomolecular Systems, Max Planck Institute for Colloids and Interfaces, Potsdam, Germany
6Institute of Chemistry and Biochemistry, Free University, Berlin, Germany
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Anne Sadewasser
7Div. of Influenza and other Respiratory Viruses, Robert Koch-Institute, 13353 Berlin, Germany
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Markus Lesch
8Molecular Biology Department, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
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Xenia Wörmann
8Molecular Biology Department, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
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Alexander Karlas
8Molecular Biology Department, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
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Peter Seeberger
5Department for Biomolecular Systems, Max Planck Institute for Colloids and Interfaces, Potsdam, Germany
6Institute of Chemistry and Biochemistry, Free University, Berlin, Germany
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Thorsten Wolff
7Div. of Influenza and other Respiratory Viruses, Robert Koch-Institute, 13353 Berlin, Germany
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Peter Hinterdorfer
1Institute for Biophysics, Johannes Kepler University Linz, 4020 Linz, Austria
2Center for Advanced Bioanalysis GmbH, A-4020 Linz, Austria
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  • For correspondence: christian.sieben@epfl.ch andreas.herrmann@rz.hu-berlin.de peter.hinterdorfer@jku.at
Andreas Herrmann
9Department of Biology, Molecular Biophysics, Humboldt Universität zu Berlin, 10115 Berlin, Germany
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  • For correspondence: christian.sieben@epfl.ch andreas.herrmann@rz.hu-berlin.de peter.hinterdorfer@jku.at
Christian Sieben
9Department of Biology, Molecular Biophysics, Humboldt Universität zu Berlin, 10115 Berlin, Germany
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  • ORCID record for Christian Sieben
  • For correspondence: christian.sieben@epfl.ch andreas.herrmann@rz.hu-berlin.de peter.hinterdorfer@jku.at
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Abstract

Influenza A viruses (IAV) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycan receptors on host cells. HAs receptor specificity towards sialic acid (SA) is well studied and clearly critical for virus infection, but the contribution of the highly complex cellular plasma membrane to the cellular specificity remains elusive. In addition, some studies indicated that other host cell factors such as the epidermal growth factor receptor might contribute to the initial virus-cell contact and further downstream signaling1.

Here we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS) to compare influenza virus receptor specificity with actual host cell binding. Unexpectedly, our study reveals that HAs receptor binding preference does not necessarily reflect virus-cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAV thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 14, 2017.
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Relevance of host cell surface glycan structure for cell specificity of influenza A virus
Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Dandan Zhang, Jianping Liu, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann, Christian Sieben
bioRxiv 203349; doi: https://doi.org/10.1101/203349
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Relevance of host cell surface glycan structure for cell specificity of influenza A virus
Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Dandan Zhang, Jianping Liu, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann, Christian Sieben
bioRxiv 203349; doi: https://doi.org/10.1101/203349

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