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F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis

Mariia Burdyniuk, Andrea Callegari, Masashi Mori, Francois Nedelec, Peter Lenart
doi: https://doi.org/10.1101/265009
Mariia Burdyniuk
European Molecular Biology Laboratory (EMBL);
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Andrea Callegari
European Molecular Biology Laboratory (EMBL);
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Masashi Mori
RIKEN Center for Developmental Biology
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Francois Nedelec
European Molecular Biology Laboratory (EMBL);
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Peter Lenart
European Molecular Biology Laboratory (EMBL);
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  • For correspondence: lenart@embl.de
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Abstract

Capture of each and every chromosome by spindle microtubules is essential to prevent chromosome loss and aneuploidy. In somatic cells, astral microtubules search and capture chromosomes forming lateral attachments to kinetochores. However, this mechanism alone is insufficient in large oocytes. We have previously shown that a contractile F-actin network is additionally required to collect chromosomes scattered in the 70-μm starfish oocyte nucleus. How this F-actin-driven mechanism is coordinated with microtubule capture remained unknown. Here, we show that after nuclear envelope breakdown Arp2/3-nucleated F-actin patches form around chromosomes in a Ran-GTP-dependent manner, and we propose that these structures sterically block kinetochore-microtubule attachments. Once F-actin-driven chromosome transport is complete, coordinated disassembly of these F-actin patches allows synchronous capture by microtubules. Our observations indicate that this coordination is necessary, as early capture of chromosomes by microtubules would interfere with F-actin-driven transport leading to chromosome loss and formation of aneuploid eggs.

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Posted February 13, 2018.
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F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis
Mariia Burdyniuk, Andrea Callegari, Masashi Mori, Francois Nedelec, Peter Lenart
bioRxiv 265009; doi: https://doi.org/10.1101/265009
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F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis
Mariia Burdyniuk, Andrea Callegari, Masashi Mori, Francois Nedelec, Peter Lenart
bioRxiv 265009; doi: https://doi.org/10.1101/265009

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