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An ancestral role of pericentrin in centriole formation through SAS-6 recruitment

View ORCID ProfileDaisuke Ito, View ORCID ProfileSihem Zitouni, View ORCID ProfileSwadhin Chandra Jana, View ORCID ProfilePaulo Duarte, View ORCID ProfileJaroslaw Surkont, Zita Carvalho-Santos, Jose B. Pereira-Leal, View ORCID ProfileMiguel Godinho Ferreira, View ORCID ProfileMonica Bettencourt-Dias
doi: https://doi.org/10.1101/313494
Daisuke Ito
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Sihem Zitouni
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Swadhin Chandra Jana
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Paulo Duarte
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Jaroslaw Surkont
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Zita Carvalho-Santos
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Jose B. Pereira-Leal
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Miguel Godinho Ferreira
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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Monica Bettencourt-Dias
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal
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  • For correspondence: mdias@igc.gulbenkian.pt
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Abstract

The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar matrix (PCM). Centrioles regulate matrix assembly. Here we ask whether the matrix also regulates centriole assembly. To define the interaction between the matrix and individual centriole components, we take advantage of a heterologous expression system using fission yeast. Importantly, its centrosome, the spindle pole body (SPB), has matrix but no centrioles. Surprisingly, we observed that the SPB can recruit several animal centriole components. Pcp1/pericentrin, a conserved matrix component that is often upregulated in cancer, recruits a critical centriole constituent, SAS-6. We further show that this novel interaction is conserved and important for centriole biogenesis and elongation in animals. We speculate that the Pcp1/pericentrin-SAS-6 interaction surface was conserved for one billion years of evolution after centriole loss in yeasts, due to its conserved binding to calmodulin. This study reveals an ancestral relationship between pericentrin and the centriole, where both regulate each other assembly, ensuring mutual localisation.

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Posted May 03, 2018.
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An ancestral role of pericentrin in centriole formation through SAS-6 recruitment
Daisuke Ito, Sihem Zitouni, Swadhin Chandra Jana, Paulo Duarte, Jaroslaw Surkont, Zita Carvalho-Santos, Jose B. Pereira-Leal, Miguel Godinho Ferreira, Monica Bettencourt-Dias
bioRxiv 313494; doi: https://doi.org/10.1101/313494
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An ancestral role of pericentrin in centriole formation through SAS-6 recruitment
Daisuke Ito, Sihem Zitouni, Swadhin Chandra Jana, Paulo Duarte, Jaroslaw Surkont, Zita Carvalho-Santos, Jose B. Pereira-Leal, Miguel Godinho Ferreira, Monica Bettencourt-Dias
bioRxiv 313494; doi: https://doi.org/10.1101/313494

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