Optogenetics harnesses natural photoreceptors to non‐invasively control selected processes
in cells with previously unmet spatiotemporal precision. Linking the activity of a protein of …

Optogenetic control of CRISPR–Cas9 systems has significantly improved our ability to perform
genome perturbations in living cells with high precision in time and space. As new Cas …

Virus-host interactions can reveal potentially effective and selective therapeutic targets for
treating infection. Here, we performed an integrated analysis of the dynamics of virus …

Abstract Domain insertion engineering is a promising approach to recombine the functions
of evolutionarily unrelated proteins. Insertion of light‐switchable receptor domains into a …

Anti-CRISPR (Acr) proteins are powerful tools to control CRISPR–Cas technologies. However,
the available Acr repertoire is limited to naturally occurring variants. Here, we applied …

Proteins are nature’s most versatile molecular machines. Deep neural networks trained on
large protein datasets have recently been used to tackle the unmet complexity of protein …

Since the breakthrough discoveries that CRISPR-Cas9 nucleases can be easily programmed
and employed to induce targeted double-strand breaks in mammalian cells, the gene …

Light-controlled transcriptional activation is a commonly used optogenetic strategy that
allows researchers to regulate gene expression with high spatiotemporal precision. The vast …

Deep mutational scanning is a powerful method to explore the mutational fitness landscape
of proteins. Its adaptation to anti-CRISPR proteins, which are natural CRISPR-Cas inhibitors …

The worldwide spread of severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2)
caused an urgent need for an in-depth understanding of interactions between the …