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Concentration Dependent Chromatin States Induced by the Bicoid Morphogen Gradient

Colleen E. Hannon, Shelby A. Blythe, Eric F. Wieschaus
doi: https://doi.org/10.1101/133348
Colleen E. Hannon
1Department of Molecular Biology/Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
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Shelby A. Blythe
1Department of Molecular Biology/Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
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Eric F. Wieschaus
1Department of Molecular Biology/Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
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  • For correspondence: efw@princeton.edu
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ABSTRACT

In Drosophila, graded expression of the maternal transcription factor Bicoid (Bcd) provides positional information to activate target genes at different positions along the anterior-posterior axis. We have measured the genome-wide binding profile of Bcd using ChIP-seq in embryos expressing single, uniform levels of Bcd protein, and grouped Bcd-bound targets into four classes based on occupancy at different concentrations. By measuring the biochemical affinity of target enhancers in these classes in vitro and genome-wide chromatin accessibility by ATAC-seq, we found that the occupancy of target sequences by Bcd is not primarily determined by Bcd binding sites, but by chromatin context. Bcd drives an open chromatin state at a subset its targets. Our data support a model where Bcd influences chromatin structure to gain access to concentration-sensitive targets at high concentrations, while concentration-insensitive targets are found in more accessible chromatin and are bound at low concentrations.

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Posted May 03, 2017.
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Concentration Dependent Chromatin States Induced by the Bicoid Morphogen Gradient
Colleen E. Hannon, Shelby A. Blythe, Eric F. Wieschaus
bioRxiv 133348; doi: https://doi.org/10.1101/133348
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Concentration Dependent Chromatin States Induced by the Bicoid Morphogen Gradient
Colleen E. Hannon, Shelby A. Blythe, Eric F. Wieschaus
bioRxiv 133348; doi: https://doi.org/10.1101/133348

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