Abstract
Both emerging viruses and well-known viral pathogens endowed with neurotropism can either impair directly the neuronal functions or induce physio-pathological changes by diffusing from the periphery through neurosensory-epithelial connections. However, the current lack of an in vitro system modeling the connectivity between human neurons and peripheral tissues excludes the analysis of viral latency and reactivation and the assessment of natural/artificial induced anti-viral immunity. In this study, we developed the first stable topographic neurosensory-epithelial connection on-a-chip using human stem cell derived dorsal root ganglia (DRG) sensory neurons. Bulk and single cell transcriptomics showed that different combinations of key receptors for Herpes Simplex Virus 1 (HSV-1) are expressed by each sensory neuronal cell type. This neuronal-epithelial circuitry enabled a detailed analysis of the HSV infectivity faithfully modeling its dynamics and cell type specificity. The reconstitution of an organized connectivity between human sensory neurons and keratinocytes into microfluidic chips provides for the first time a powerful in vitro platform to model viral latency and reactivation of human viral pathogens.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
↵§ These authors jointly supervised this work.
↵✉ e-mail: clementi.nicola{at}hsr.it; broccoli.vania{at}hsr.it
Summary In vitro studies on HSV-1 reactivation from latency remain very challenging for the current lack of human models reconstituting the neuroepithelial connectivity. Taking advantage of DRG-like organoids, here it is described a new stem cell-based platform on-a-chip which recapitulates the neuron-to-keratinocyte connection where the dynamics and kinetics of HSV reactivation can be fully elucidated.