Abstract
We present a 1.3-μm reflection matrix microscope and computational conjugate adaptive optics algorithm for label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks down to cortical layer 4 with a near-diffraction-limited resolution of 0.79 µm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.
Competing Interest Statement
The authors have declared no competing interest.
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