Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

HSA+ immature cardiomyocytes persist in the adult heart and expand after ischemic injury

View ORCID ProfileMariana Valente, View ORCID ProfileTatiana Pinho Resende, View ORCID ProfileDiana Santos Nascimento, Odile Burlen-Defranoux, Benoit Dupont, View ORCID ProfileAna Cumano, View ORCID ProfilePerpétua Pinto-do-Ó
doi: https://doi.org/10.1101/518530
Mariana Valente
1i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
2INEB – Instituto Nacional de Engenharia Biomédica, Universidade do Porto, 4200-135,Porto, Portugal.
3ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313, Porto, Portugal.
4Unit for Lymphopoiesis, Immunology Department, INSERM U1223, Institut Pasteur, 75015, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mariana Valente
Tatiana Pinho Resende
1i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
2INEB – Instituto Nacional de Engenharia Biomédica, Universidade do Porto, 4200-135,Porto, Portugal.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tatiana Pinho Resende
Diana Santos Nascimento
1i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
2INEB – Instituto Nacional de Engenharia Biomédica, Universidade do Porto, 4200-135,Porto, Portugal.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Diana Santos Nascimento
Odile Burlen-Defranoux
4Unit for Lymphopoiesis, Immunology Department, INSERM U1223, Institut Pasteur, 75015, Paris, France.
5Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, 75018, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benoit Dupont
6Beckman Coulter, France S.A.S, 93420 Villepinte, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ana Cumano
4Unit for Lymphopoiesis, Immunology Department, INSERM U1223, Institut Pasteur, 75015, Paris, France.
5Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, 75018, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ana Cumano
Perpétua Pinto-do-Ó
1i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
2INEB – Instituto Nacional de Engenharia Biomédica, Universidade do Porto, 4200-135,Porto, Portugal.
3ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313, Porto, Portugal.
4Unit for Lymphopoiesis, Immunology Department, INSERM U1223, Institut Pasteur, 75015, Paris, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Perpétua Pinto-do-Ó
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The assessment of the regenerative capacity of the heart has been compromised by the lack of surface signatures to characterize cardiomyocytes. Here, combined multiparametric surface marker analysis with single cell transcriptional profiling and in vivo transplantation, identify the main fetal cardiac populations and their progenitors. We found that cardiomyocytes at different stages of differentiation co-exist during development. We identified a population of immature HSA/CD24+ cardiomyocytes that persists throughout life and that, unlike other cardiomyocyte subsets, actively proliferates up to one week of age and engraft cardiac tissue upon transplantation. In adult heart HSA/CD24+ cardiomyocytes appear as mononucleated cells that cycle and increase in frequency after infarction. Our work identified cell surface signatures that allow the prospective isolation of cardiomyocytes at any developmental stage and the detection of adult cardiomyocytes poised for activation in response to ischemic stimuli. This work opens new perspectives in the understanding and treatment of heart pathologies.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 13, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
HSA+ immature cardiomyocytes persist in the adult heart and expand after ischemic injury
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
HSA+ immature cardiomyocytes persist in the adult heart and expand after ischemic injury
Mariana Valente, Tatiana Pinho Resende, Diana Santos Nascimento, Odile Burlen-Defranoux, Benoit Dupont, Ana Cumano, Perpétua Pinto-do-Ó
bioRxiv 518530; doi: https://doi.org/10.1101/518530
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
HSA+ immature cardiomyocytes persist in the adult heart and expand after ischemic injury
Mariana Valente, Tatiana Pinho Resende, Diana Santos Nascimento, Odile Burlen-Defranoux, Benoit Dupont, Ana Cumano, Perpétua Pinto-do-Ó
bioRxiv 518530; doi: https://doi.org/10.1101/518530

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2635)
  • Biochemistry (5224)
  • Bioengineering (3650)
  • Bioinformatics (15730)
  • Biophysics (7218)
  • Cancer Biology (5597)
  • Cell Biology (8051)
  • Clinical Trials (138)
  • Developmental Biology (4739)
  • Ecology (7472)
  • Epidemiology (2059)
  • Evolutionary Biology (10531)
  • Genetics (7704)
  • Genomics (10090)
  • Immunology (5157)
  • Microbiology (13829)
  • Molecular Biology (5354)
  • Neuroscience (30605)
  • Paleontology (212)
  • Pathology (872)
  • Pharmacology and Toxicology (1520)
  • Physiology (2235)
  • Plant Biology (4987)
  • Scientific Communication and Education (1037)
  • Synthetic Biology (1380)
  • Systems Biology (4131)
  • Zoology (804)